{"id":1727,"date":"2019-01-19T00:04:21","date_gmt":"2019-01-19T00:04:21","guid":{"rendered":"http:\/\/ladam.info\/?p=1727"},"modified":"2019-01-19T00:04:21","modified_gmt":"2019-01-19T00:04:21","slug":"low-cost-modular-multi-strain-diagnostic-ongoing","status":"publish","type":"post","link":"https:\/\/ladam.info\/index.php\/2019\/01\/19\/low-cost-modular-multi-strain-diagnostic-ongoing\/","title":{"rendered":"Low-cost, modular, multi-strain diagnostic (ongoing)"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">This is the application part of the <a href=\"https:\/\/ladam.info\/index.php\/2019\/01\/18\/synthetic-control-of-yeast-culture-growth-rates-ongoing\/\">project I am finishing<\/a>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">We engineered yeast to induce growth arrest using a number of genes\/inducers that we then characterized. We then used them as a <strong>diagnostic tool<\/strong>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"> We used <strong>nonlinear differential equations<\/strong>. We model our co-culture experiments and made predictions.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">We used a <strong>flow cytometer<\/strong> to collect our data. We also took <strong>images <\/strong>of our system as we used a yeast strain that turns purple in some cases so I did some image processing of our pretty colored colonies.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">All the code and data will be release with the publication in <strong>github<\/strong> and ebioome. Specifically, I used python (<strong>opencv,<\/strong> <strong>pandas, seaborn, lmfit, scikit-learn<\/strong>). <\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n<p><!--EndFragment--><br>\n<br>\n<\/p>","protected":false},"excerpt":{"rendered":"<p>This is the application part of the project I am finishing. We engineered yeast to induce growth arrest using a number of genes\/inducers that we then characterized. We then used them as a diagnostic tool. We used nonlinear differential equations. We model our co-culture experiments and made predictions. We used a flow cytometer to collect&nbsp;&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"nf_dc_page":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[263,262,264,258,254,274],"tags":[],"class_list":["post-1727","post","type-post","status-publish","format-standard","hentry","category-analytics","category-modeling","category-prediction","category-python","category-synthetic-biology","category-yeast-diagnostic"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/posts\/1727","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/comments?post=1727"}],"version-history":[{"count":1,"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/posts\/1727\/revisions"}],"predecessor-version":[{"id":1728,"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/posts\/1727\/revisions\/1728"}],"wp:attachment":[{"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/media?parent=1727"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/categories?post=1727"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ladam.info\/index.php\/wp-json\/wp\/v2\/tags?post=1727"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}